Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Schematic overview of the EIS aptasensor for the simultaneous detection of MPT64 and S-glycoprotein.

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Electrochemical surface characterization of the SPGE using CV: (A) MPT64 and (B) S-glycoprotein at 1 pg/mL. Nyquist plots of stepwise characterization of the SPGE using EIS: (C) MPT64 and (D) S-glycoprotein. The Randles circuit was used to model the EIS data (E) , where R s is the solution resistance, R ct is the charge-transfer resistance, W is the Warburg impedance, and CPE is the constant phase element.

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Sensitivity analysis of the EIS aptasensor for the simultaneous detection of (A) MPT64 and (B) S-glycoprotein in buffer and human serum. The insets display the linear calibration curves corresponding to the detection of (A) MPT64 and (B) S-glycoprotein in the working buffer and spiked human serum. The data are presented as the means of three independent measurements.

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Specificity of the developed EIS aptasensor for simultaneous detection of MPT64 and S glycoprotein, with (A) WE 1 functionalized with the MPT64 aptamer and (B) WE 2 functionalized with the S glycoprotein aptamer. The target and nontarget proteins were tested at a concentration of 1 pg/mL. The data are presented as the means ± SEMs (*0.01 < p < 0.05). Stability of the EIS aptasensor for dual antigen detection of (C) MPT64 and (D) S-glycoprotein at a 1 pg/mL concentration at 4 °C. The data are presented as the means ± SEMs, with n = 3 independent replicates for each day ( ns –nonsignificant).

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques: Concentration Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Contact angle measured at various stages of EIS aptasensor fabrication for the simultaneous detection of two antigens: (A) MPT64 and (B) S-glycoprotein. ** 0.001 < p < 0.01, **** 0.00001 < p < 0.0001 (one-way ANOVA). The experiments were conducted with three biological replicates. All the data are presented as the means ± SEMs.

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensor

doi: 10.3389/fbioe.2025.1692839

Figure Lengend Snippet: Analysis of the surface morphology of the bare and functionalized electrodes. Representative 3D images of a 0.16 μm 2 scanned electrode surface for (A) a bare electrode, (B) functionalization with an S glycoprotein aptamer, (C) blocking with MCH (S glycoprotein), (D) binding with S-glycoprotein, (E) functionalization with an MPT64 aptamer, (F) blocking with MCH (MPT64), and (G) binding with MPT64. (H) Comparison of roughness for all functionalization steps of the S glycoprotein electrode and (J) the MPT64 electrode (N ≥ 31). (I) Comparison of height differences for all functionalization steps of the S glycoprotein electrode and (K) the MPT64 electrode.

Article Snippet: The SARS-CoV-2 S glycoprotein (cat. no. 40592-VNAH), monkey pox virus (MPXV) A29 protein (cat. no. 40891-V08E), and Middle East respiratory syndrome-related coronavirus (MERS-CoV) S glycoprotein (cat. no. 40071-V08B1) were purchased from Sino Biological (China).

Techniques: Blocking Assay, Binding Assay, Comparison